《植物生理学报》 2013, 49(12): 1347-1354

粳稻穗部结构性状的QTL分析

姜树坤^1,2, 王嘉宇³, 姜辉¹, 洛育¹, 孙世臣¹, 张喜娟^1,2, 白良明¹, 陈温福³, 张凤鸣^1,*, 徐正进³

¹黑龙江省农业科学院耕作栽培研究所, 哈尔滨150086; ²中国科学院北方粳稻分子育种联合研究中心; 哈尔滨150086; ³沈阳农业大学水稻研究所, 沈阳110866

通信作者：张凤鸣；E-mail: zhangfengming570@163.com；Tel: 0451-86680041

摘　要：

利用温带粳稻‘沈农265’和‘丽江新团黑谷’构建的重组自交系群体, 在沈阳和哈尔滨两地对15个穗部结构性状进行了QTL分析。共检测到61个相关QTL, 其中沈阳检测到的38个QTL在第1、4、6、11和12号染色体上形成了5个QTL簇; 而在哈尔滨检测到的31个QTL也在第3、9和10号染色体上形成了QTL簇。仅有8个QTL是在两地同时被检测到的, 分别是控制一次枝梗数的qPBN4、控制穗长的qPL6和qPL9、控制一次枝梗实粒数的qGNPB4、控制一次枝梗颖花数的qTSNPB6.1、控制结实率的qPSS10、以及控制着粒密度的qSD3和qSD9。其中, qPBN4 (最高表型贡献率43.2%)、qPL9 (最高表型贡献率63.2%)、qGNPB4 (最高表型贡献率30.9%)和qSD9 (最高表型贡献率42.9%)是主效QTL。通过进一步的分析发现控制穗长qPL9和控制着粒密度qSD9位于DEP1所在区间。同时控制一次枝梗数和一次枝梗实粒数的位于第4号染色体长臂端的穗部结构主效QTL, qPBN4~qGNPB4极富研究与应用价值。

关键词：水稻; 穗部结构; 数量性状基因位点

收稿：2013-06-09   修定：2013-08-06

资助：中国博士后科学基金特别资助(2013T60396)、国家科技支撑计划(2011BAD16B11)和国家科技支撑计划(2011-BAD35B02)。

Dissection of QTLs for Panicle Structure Traits in Rice (Oryza sativa L. spp. japonica)

JIANG Shu-Kun^1,2, WANG Jia-Yu³, JIANG Hui¹, LUO Yu¹, SUN Shi-Chen¹, ZHANG Xi-Juan^1,2, BAI Liang-Ming¹, CHEN Wen-Fu³, ZHANG Feng-Ming^1,*, XU Zheng-Jin³

¹Cultivation and Farming Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; ²Northern Japonica Rice Molecular Breeding Joint Research Center, Chinese Academy of Sciences, Harbin 150086, China; ³Rice Research Institution, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: ZHANG Feng-Ming; E-mail: zhangfengming570@163.com; Tel: 0451-86680041

Abstract:

Using recombinant inbred lines (RILs) derived from a cross between temperate japonica rice cultivars, ‘Shennong265’ (SN265) and ‘Lijiangxintuanheigu’ (LTH), we identified 61 quantitative trait loci (QTL) for 15 panicle structure characteristics on the all 12 chromosomes in Shenyang and Harbin. The 38 QTLs in Shenyang formed five QTL-clusters on chromosome 1, 4, 6, 11 and 12. The QTLs identified in Harbin showed three QTLclusters on chromosome 3, 9 and 10. Only eight from total 61 QTLs were detected at two locations. They were qPBN4 controlling primary branch number; qPL6 and qPL9 controlling panicle length; qGNPB4 for grains number for primary branch;qTSNPB6.1 for total spikelet number of primary branch; qPSS10 controlling percent seed set and qSD3 and qSD9 controlling seed density. Among them, qPBN4 (higher phenotypic variation were 43.2%), qPL9 (higher phenotypic variation were 63.2%), qGNPB4 (higher phenotypic variation were 30.9%) and qSD9 (Higher phenotypic variation were 42.9%) were major QTLs. Further analysis showed that the loci qPL9 and qSD9 was located near DEP1. qPBN4–qGNPB4 for panicle structure traits on long arm of chromosome 4 for primary branch number and primary branch grains number have a high research and application value.

Key words: rice (Oryza sativa L. spp. japonica); panicle structure; quantitative trait loci